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Journal: Frontiers in Cellular and Infection Microbiology
Article Title: Biological boundary conditions regulate the internalization of Aspergillus fumigatus conidia by alveolar cells
doi: 10.3389/fcimb.2025.1515779
Figure Lengend Snippet: Internalization of A fumigatus conidia in a subpopulation of Lamp + Actin + vesicles in cells on circular micropatterns and effect of ECM composition on conidia internalization. (A) Representative example of two cells on fibronectin-coated circular micropattern (28 µm). Overview of Lamp1 and Actin is shown as maximal Z-projection of FITC/TRITC channels of 9 focal planes. Magnified images show conidia in Lamp1 + (green) and Lamp1 + Actin + vesicles (red and green) 3 hours post-infection. Scale bars = 10 µm. (B) Percentage of conidia internalized in Lamp1 + Actin + vesicles relative to the total conidia number in cells constrained on fibronectin-coated circular micropatterns with different sizes and cell density at 1 and 3 hours post-infection. (C) Conidia spatial distribution map of 50 overlaid micropatterns displaying Lamp1 + Actin + vesicles with internalized conidia (green spheres in red circles). (D) Comparison of vitronectin- and fibronectin micropatterns. Quantification of the average number of total conidia and the percentage of conidia internalized in Lamp1 + and Lamp1 + Actin + vesicles at 1 and 3 hours post-infection relative to the total conidia number. **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001; ns P > 0.05. VN, vitronectin; FN, fibronectin. See also Supplementary Figure 1 .
Article Snippet: The patterned substrates were subsequently incubated with 150 μl of fibronectin (10 μg/ml, Sigma Aldrich, 341631, Austria) in PBS or vitronectin (3.3 μl/ml, ProSci, 91-362, USA) at 4°C overnight and washed once with PBS.
Techniques: Infection, Comparison